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Figure 5 | BMC Plant Biology

Figure 5

From: In Medicago truncatula, water deficit modulates the transcript accumulation of components of small RNA pathways

Figure 5

miR162 and miR168 expression in shoots and roots of M. truncatula in different water status. The U6 small nuclear RNA was used as internal loading control. The accumulation of miR162 and miR168 (numbers indicated under each lane) was quantified according to U6 small nuclear RNA loading control and normalized to control conditions. The membrane was first hybridized with miR162 probe and then striped and rehybridized with miR168 probe. (M) miRNA size marker with three bands of 24, 21 and 17 nt (New England Biolabs) is shown in the left.. Ct, Control; MWD, Moderate Water Deficit; SWD, Severe Water Deficit; Rec, Recovery.

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