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Figure 3 | BMC Plant Biology

Figure 3

From: Characterization of cp3 reveals a new bri1 allele, bri1-120, and the importance of the LRR domain of BRI1 mediating BR signaling

Figure 3

Cp3 is allelic to bri1. A. The schematic protein structure of BRI1 is shown and the mutation site of bri1-120/cp3 is marked in the 13th LRR of the extracellular domain of BRI1. B. Overexpression of BRI1 rescued the cp3 mutant phenotypes. The pictures were taken of 3-week-old plants (left panel) and 5-week-old plants (right panel). C. RT-PCT analysis shown the expression of endogenous BRI1 and the BRI1 derived from the transgene in the cp3 mutant overexpressing BRI1 compared with those of Ler and un-transformed cp3 mutant. D. Quantitative growth criteria were measured from the transgenic cp3 overexpressing BRI1. LL: leaf length, LW: leaf width, PL: petiole length, and H: total height. Growth is represented as a relative value compared to that of Ler. Experiments were repeated twice. Error bars denote standard errors. E. Pattern of transcriptional inhibition of CPD expression in response to BL was restored in the transgenic cp3 overexpressing BRI1.

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