Figure 4

Analysis of seed protein extracts from Ara h 2.02 knockout mutant. A - Coomassie blue stained SDS-PAGE of seed protein extracts, with equal amounts of total protein loaded in each lane. Lane wt: wild type (Tifrunner). Lane 1: homozygous mutant. Lane 2: heterozygote. B - Western blot of seed protein extracts using anti-Ara h 2 antibodies, which recognize both isoforms of the allergen. Antibodies also recognize Ara h 6. Lane wt: wild type (Tifrunner). Lane 1: homozygous mutant. Lane 2: heterozygote. C - 2D DIGE analysis of seed protein extracts from wild-type (Tifrunner) labeled with Cy3 (green) and Ara h 2.02 knockout mutant labeled with Cy2 (red). The white box denotes the four spots representing Ara h 2 isoforms.