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Table 1 Summary of amplicon sizes and frequencies of mutations identified by TILLING in two different EMS treatments

From: TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.)

 

Amplicon

Screened

0.4% EMS/12 hr.

1.2% EMS/4.5 hr.

Total

Gene

bp

No. of Mutations:

Ara h 1.01

2211

2011

2

2

4

Ara h 1.02

1666

1466

1

0

1

Ara h 2.01

1278

1078

7

2

9

Ara h 2.02

1226

1026

2

3

5

Ah FAD2A

1228

1028

5

0

5

Ah FAD2B

1221

1021

3

0

3

Total:

8830

7630

20

7

27

  

Plants Screened:

2441

979

3420

  

kb/SNP:

931

1067

966

  1. For number of bp screened, 200 bp is subtracted to adjust for the 100-bp regions at the top and bottom of TILLING gel images that are difficult to analyze.