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Figure 1 | BMC Plant Biology

Figure 1

From: Identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape (Vitis viniferaL.) based on curation and mining of large-scale EST data sets

Figure 1

Correcting erroneous EST identities in bi-directionally sequenced leaf and berry libraries with dot plots. Contig names assigned to ESTs from bi-directionally sequenced libraries were plotted in two dimensions to identify "motifs of self-similarity" analogous to dot-plot sequence alignments. The sequencing batch, plate order, and well position were recapitulated from dbEST submission files as a sequential list arranged as 1f, 1r, 2f, 2r, 3f, 3r, 4f, 4r, and plotted against itself in the x and y axes. A) Diagonals indicate four sets of plates from Library ID 12948, batch 8 are named and paired correctly (blue); B) Library ID 12753, batch 1, all combinations of plates 1f, 1r, 2f and 2r are duplicates (salmon), plates 3 and 4 are correctly paired (blue); C) Library ID 12948, batch 10 plate 1f matches 1r (blue), plate 2f and 2r did not match, plate 3f matches 4r (salmon), 4f matches 3r (magenta); D) Berry Library ID 13016, batch 1, plate 3r matches with 2f and 2r (salmon), 1r matches with 3f (magenta), 1f has no match, plate 4 is paired correctly (blue); E) Library ID 13017, batch 2, Plates 1 and 2 display partial matching (pink), plates 2 and 3 also partially match (purple); F) Berry Library ID 13017 batch 3, partial matching between all four plates (purple); G) Berry Library ID 13015, batch 2, plate 1 matches batch 5 plate 1r (salmon); other plate match errors are also apparent in lower right hand quandrant (magenta); H), Leaf Library ID 12752, batch 5, plate 4r matches Berry Library ID 12754, batch 5, plates 4fr (salmon).

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