Identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape (Vitis viniferaL.) based on curation and mining of large-scale EST data sets

Table 2 Correction of errors in the identifications of ESTs in a set of libraries

Error category	Specific error type	# of Errors	See also
Well pairing "slips"
Leaf Library ID 12948		10	Figure 1A
Leaf Library ID 12949		1
Incorrect plate pairings
Leaf Library ID 12753	Plate quadruplicated	1	Figure 1B
Berry Library ID 12754	Plate quadruplicated	1
Leaf Library ID 12948	Plate pair swap	2	Figure 1C
Berry Library ID 13015	Plate pair swap	2
	Plate triplicated	3	Figure 1G
Berry Library ID 13016	Plate triplicated	1	Figure 1D
	Plate pair swap	1
Partial plate duplications
Berry Library ID 13016		6
Berry Library ID 13017		28	Figure 1E, 1F
Sequences originated from a different library
Leaf Library ID 12752 and Berry Library ID 12754	Plate of "leaf" ESTs actually a triplicate of berry Lib.12754 ESTs	1	Figure 1H

Errors in the supplied annotation of a set of cDNA clones that were sequenced bidirectionally were identified and corrected to generate accurate counts of EST frequency. Errors are categorized by the scope of the error, from "well slips" between single pairs of 5' and 3' 96-well plates of ESTs, through incorrectly identified pairs of plates of increasing scope. The number of times each error occurred (pairs or larger groups of 96-well plates affected) and was corrected is shown. Errors that are visualized by dot-plot in Figure 1 are cross-referenced.

ISSN: 1471-2229