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Table 3 List of the spots/proteins identified by mass spectrometry.

From: Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin

GO

Spot ID

Protein (Species)

GB

Score

% Cv

Mw (kD) (Exp/Theo)

pI (Exp/The)

Pep-tides

CPT/CON

Stress responses

 

U1

Chloroplast Cu/Zn superoxide dismutase (maize)

NP001108127

128

24

17.51/20.99

4.71/5.45

2

CPT

 

U2

Cytosolic ascorbate peroxidase (maize)

ACG38188

114

11

26.76/27.46

5.35/5.55

3

3.25

 

U3

Glutathione S-transferase 19 (maize)

AAG34827

78

12

24.60/25.40

6.37/7.68

2

CPT

Defense

 

D1

Pathogenesis-related protein 1 (maize)

ACG29538

150

34

17.10/17.07

4.98/5.39

3

CON

 

D2

Bet v I allergen (maize)

AY754698

66

24

18.30/17.07

3.80/4.68

3

CON

Protein degradation

 

D3

26S proteasome non-ATPase regulatory subunit 4 (maize)

ACG37494

138

6

60.32/42.4

3.54/4.46

2

CON

 

U4

26S proteasome non-ATPase regulatory subunit 8 (maize)

ACG40420

138

14

29.87/31.05

4.70/5.03

3

CPT

Protein synthesis

 

D4

Eukaryotic translation initiation factor eIF-5A (maize)

CAA69225

197

70

20.01/17.70

5.38/5.61

7

CON

 

D5

Eukaryotic translation initiation factor eIF-6 (maize)

ACG33598

92

6

26.93/27.01

3.95/4.64

1

0.46

DNA replication, recombination & repair

 

D6

Histone H2B (maize)

CAA40564

48

12

19.55/16.41

5.75/10.00

2

CON

RNA metabolism

 

D7

RraA (rice)

NP916709

101

26

19.01/18.06

5.35/6.12

4

0.5

 

U5

RraA (maize)

ACG30537

307

39

18.58/18.19

4.95/5.33

6

CPT

 

D8

DEAD-family RNA helicase (rice)

NP921002

128

30

27.72/26.60

6.46/6.82

5

CON

 

U6

Glycine-rich RNA-binding protein 8 (maize)

ACG35695

70

8

23.60/21.38

7.00/7.63

2

CPT

 

D9

RNA recognition motif containing protein (maize)

ACN31194

150

15

32.33/30.23

9.01/8.57

3

0.87

Metabolism

 

D10

Cytosolic GAPDH (maize)

CAA51676

70

4

20.19/36.63

5.88/6.46

1

CON

 

U7

Glyceraldehyde-3-phosphate dehydrogenase (Arabidopsis)

AAK15554

74

14

40.95/44.97

7.24/8.75

4

3.23

 

D11

Glyceraldehyde-3-phosphate dehydrogenase (Arabidopsis)

JQ1287

78

4

20.19/37.07

5.88/6.34

4

CON

 

D12

Triosephosphate isomerase 1 (maize)

AAB81110

139

40

25.94/27.24

5.34/5.52

8

CON

Signalling

 

U8

Protein-G β-subunit (rice)

NP916988

100

21

36.34/36.66

5.86/5.97

4

2.84

Endomembrane trafficking

 

D13

Syntaxin 6 (rice)

NP001065843

121

42

32.32/24.10

6.70/5.56

7

CON

Energy

 

U9

Mitochondrial ATP synthase precursor (maize)

ACG38121

84

11

29.87/25.57

4.7/7.71

2

CPT

Embryo and seed storage

 

U10

Vicilin (rice)

CAA41810

86

15

14.12/65.45

5.00/6.63

5

CPT

 

U11

Vicilin (rice)

CAA41810

132

21

19.48/65.45

4.99/6.63

7

CPT

 

U12

Vicilin (rice)

CAA41810

110

8

22.12/65.45

4.77/6.63

3

CPT

 

U13

Vicilin (rice)

CAA41810

75

2

14.2/66.63

9.3/6.23

1

CPT

 

U14

globulin 2 (maize)

1802402A

64

3

15.03/50.23

6.03/6.16

1

CPT

 

D14

globulin 2 (maize)

1802402A

66

2

17.93/50.23

6.64/6.16

1

CON

 

U15

globulin 2 (maize)

1802402A

90

16

21.94/50.23

6.95/6.16

6

2.37

Unknown

 

D15

cupin domain containing protein (rice)

EAY88907

68

3

20.19/68.6

5.21/5.74

1

CON

 

U16

r40c1 protein (rice)

ACF87898

71

3

19.6/38.77

6.2/6.3

1

CPT

  1. U, proteins up-regulated by CPT. D, proteins down-regulated by CPT. GB, protein accession number in GenBank. Cv, coverage percentage. Mw/pI, theoretical and experimental molecular weight and isoelectric point. Peptides, tryptic peptides number. Changes in the relative spot volume are represented CPT/CON ratio of the mean from three independent replicas. CPT means presence only in treated samples, and CON presence only in control samples. The proteins were classified according to GO functional categories.