Figure 7From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus culturesRegeneration of transformed coffee plants from maintained embryogenic cultures. Calli in selective media containing 100 mg.L-1hygromycin and 125 mg.L-1 cefotaxime 4 months after co-cultivation with A. tumefaciens LBA1119 without plasmid, used as control (A). Regeneration of resistant calli in selective media containing 100 mg.L-1hygromycin and 125 mg.L-1 cefotaxime 4 months after co-cultivation with A. tumefaciens LBA1119 carrying pMDC32; the yellow calli are resistant to hygromycin (B). Regeneration of torpedo-shaped somatic embryos 6 months after co-cultivation (C). In vitro plantlet development 8 months after co-cultivation (D). Transgenic plants in the greenhouse 12 months after co-cultivation (E).Back to article page