Figure 8From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus culturesPCR detection of the HPTII hygromycin resistance gene in transgenic coffee plants. The plants were produced from 7-month-old embryogenic callus cultures co-cultivated with A. tumefaciens strain LBA1119 carrying out the pMDC32 binary vector. M - Molecular weight DNA markers (1 kb), C - untransformed coffee plant (control), 1-19 transgenic coffee plants derived from independent transformation events, B - blank, PCR mix without DNA, P - plasmid. The arrow indicates the fragment corresponding to the HPTII gene (506 bp).Back to article page