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Figure 8 | BMC Plant Biology

Figure 8

From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

Figure 8

PCR detection of the HPTII hygromycin resistance gene in transgenic coffee plants. The plants were produced from 7-month-old embryogenic callus cultures co-cultivated with A. tumefaciens strain LBA1119 carrying out the pMDC32 binary vector. M - Molecular weight DNA markers (1 kb), C - untransformed coffee plant (control), 1-19 transgenic coffee plants derived from independent transformation events, B - blank, PCR mix without DNA, P - plasmid. The arrow indicates the fragment corresponding to the HPTII gene (506 bp).

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