Table 2 Effect of different co-cultivation factors on transformation efficiency using established embryogenic callus cultures
Co-cultivation factors | Treatments | No. of co-cultivated calli | No. of transformed calli | Transformation efficiency (%) |
|---|---|---|---|---|
Agrobacterium conc. (OD600) | 1 (OD600 = 0.6) | 80 | 10 | 12.5 ± 11.1 |
|  | 1/10 | 102 | 2 | 1.9 ± 4.1 |
Co-cultivation temperature (°C) | 20 | 215 | 10 | 4.7 ± 4.3 |
|  | 27 | 350 | 2 | 0.6 ± 1.2 |
- *The transformation efficiency was estimated by the proportion (p) of transformed calli (p = x/n), where x was the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula
with a level of confidence of 99%
with a level of confidence of 99%