Table 3 Effect of mineral salt concentrations in the embryogenic culture proliferation medium on callus growth and transformation efficiency
Mineral salt concentration in the ECP proliferation media | Callus growth (mg/month) | No. of co-cultivated calli | Transformation efficiency (%) |
|---|---|---|---|
MS/4 | 103 ± 20.7 b* | 87 | 15.6 ± 6.6** |
MS/2 | 267 ± 24.1 a | 160 | 10.5 ± 14.1 |
MS | 254 ± 60.5 a | 100 | 0 |
1.5MS | 217 ± 53.6 a | 160 | 0 |
- * Callus growth was measured by the difference between the final and initial weight of embryogenic cultures after a 1 month proliferation cycle. The initial weight was calibrated at 120 ± 10 mg. Each datum corresponds to the mean ± SD of 4 measurements. We performed an ANOVA followed by the Tukey HSD test to identify significant differences between the means of all treatments. Values with different letters are significantly different at P ≤ 0.05. ** Transformation efficiency was analyzed by the proportion (p) of transformed calli (p = x/n), where x is the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula
with a level of confidence of 99%. Each value is the mean of 4 replicates.
with a level of confidence of 99%. Each value is the mean of 4 replicates.