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Figure 3 | BMC Plant Biology

Figure 3

From: Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca2+influx and modulates generation of reactive oxygen species in cultured rice cells

Figure 3

Effect of OsMCA1 -overexpression on Ca2+ uptake in cultured rice cells. Open and closed bars represent GUS-expressing control line (GUS No.11) and OsMCA1-overexpressing line (OX No. 2), respectively. (A) Quantification of OsMCA1 mRNA by real-time quantitative PCR. The amount of OsMCA1 mRNA was calculated from the threshold point in the log-linear range of the RT-PCR. The relative level of OsMCA1 mRNAs in the GUS-expressing control line was standardized as 1. (B) 45Ca2+ uptake into cultured rice cells. Data are means ± SD, n = 3 independent samples. *P < 0.05; significantly different compared with the control.

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