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Figure 4 | BMC Plant Biology

Figure 4

From: Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca2+influx and modulates generation of reactive oxygen species in cultured rice cells

Figure 4

Effects of OsMCA1 suppression on growth and development in planta. (A) RT-PCR analysis of OsMCA1 in five independent RNAi lines. Expression of GUS linker indicates RT-PCR products of GUS linker region, and expression of trigger dsRNA. Actin cDNA was used as internal control; PCR products were analyzed by agarose gel electrophoresis. (B) Phenotype of OsMCA1-suppressed lines of 3-week-old plants. Bars indicate 10 cm. (C) Heights of plants shown in (B) were quantified. Data are means ± SD, n = 6-13 independent plants. *P < 0.05; **P < 0.01; significantly different compared with two control lines (WT and Control 20-5). (D , E) Phenotype of OsMCA1-suppressed lines grown for 4 months (D) and 100 days (E) in a greenhouse. Arrowheads indicate ears; bars indicate 10 cm.

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BMC Plant Biology

ISSN: 1471-2229

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