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Figure 8 | BMC Plant Biology

Figure 8

From: Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca2+influx and modulates generation of reactive oxygen species in cultured rice cells

Figure 8

Effects of the expression level of OsMCA1 on hypo-osmotic shock-induced ROS generation. (A) Effects of OsMCA1-suppression on hypo-osmotic shock-induced ROS generation measured by MCLA chemiluminescence. Data are the mean ± SE for five independent experiments for the control line (closed diamond for control 20-5) and two independent RNAi lines (open circle for RNAi 20-2; open triangle for RNAi 38-5) are shown. As a hypo-osmotic shock, growth medium was replaced by four-fold diluted medium at 0 min (A and B). (B) The effect of several Ca2+ channel blockers on hypo-osmotic shock-induced ROS generation in the wild type. GdCl3 (1 mM) and LaCl3 (1 mM) were added to the cells 60 min prior to hypo-osmotic shock treatment. Data are the mean ± SE for three or four independent experiments. ***P < 0.005; significantly different compared with the control. (C) Quantitative expression levels of OsMCA1 mRNAs in the OsMCA1 overexpressor lines by real-time quantitative PCR. The relative level of the OsMCA1 mRNAs in the control cells (GUS No. 7) was standardized as 1. (D) Effects of OsMCA1 overexpression on hypo-osmotic shock-induced ROS generation. As a hypo-osmotic shock, growth medium was replaced by three-fold diluted medium at 0 min. Data are the mean ± SE for five independent experiments for two control lines (open circle for GUS No. 11; open triangle for GUS No. 7) and two overexpressor lines (closed diamond for OX No. 2; closed square for OX No. 3) are shown. *P < 0.05; **P < 0.01; significantly different compared with the control line (GUS No. 7).

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