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Figure 3 | BMC Plant Biology

Figure 3

From: The cytological changes of tobacco zygote and proembryo cells induced by beta-glucosyl Yariv reagent suggest the involvement of arabinogalactan proteins in cell division and cell plate formation

Figure 3

Localization of AGPs by immunofluorescence labeling with JIM13 in zygotes and proembryos. (A 1 -H 1 ) The fluorescence images of immunolabeling with anti-AGP antibody JIM13 under a fluorescence microscope. The antibodies labeled both cell surfaces and cell plates of zygote and proembryos in vivo (A 1 -D 1 ). In the β-GlcY treated proembryos, the immunofluorescences are remarkably weakened, especially in the cell plates (E1-G1). Under β-GlcY treatment, the cell plates of proembryos are stained into red (E 2 -G 2 ). (H1) The immunolabeling signals in the untreated proembryos in vitro are similar to that of in vivo ones. (A2-H2) Bright-field images corresponding to A 1 -H 1 . (A 3 -H 3 ) Fluorescence images of DAPI-stained nuclei corresponding to bright-field images. Bars = 10 μm.

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