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Figure 5 | BMC Plant Biology

Figure 5

From: The cytological changes of tobacco zygote and proembryo cells induced by beta-glucosyl Yariv reagent suggest the involvement of arabinogalactan proteins in cell division and cell plate formation

Figure 5

Distribution of high-esterified pectins by immunolabeling with JIM7 in zygotes and proembryos. (A 1 -G 1 ) The fluorescence images of immunolabeling with high-esterified pectins antibody JIM7 under a fluorescence microscope. The immunofluorescence gradually localizes in the apical end of cell wall during zygotes divide into proembryos in vivo (A 1 -D 1 ). In in vitro samples, high level immunofluorescence distributes in the whole cell walls of proembryos cultured with 50 μM β-GlcY (E1-F1) and the samples cultured without β-GlcY (G 1 ). (A 2 -G 2 ) Bright-field images of zygotes and proembryos corresponding to A 1 -G 1 . (A 3 -G 3 ) Fluorescence images of DAPI-stained nuclei corresponding to bright-field images. Bars = 10 μm.

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