Figure 12

Flow cytometry analysis of the ploidy level of representative diploid (control), haploid and spontaneous doubled haploid plants regenerated from microspore embryogenesis cultures. Ploidy levels were determined from propidium iodide stained-nuclei isolated from leaf discs of 4–5 leaf growth stage plants. Flow cytograms generated from a diploid donor plant (a), a haploid (b), and a spontaneous doubled haploid (c) plant regenerated from microspore cultures respectively. In the three histograms, mean channel values are presented for peaks corresponding to G1 and G2 phases of cell cycle. In the diploid control (a) and the spontaneous doubled haploid (c), G1 and G2 peaks appear at similar DNA content channels, whereas in the haploid sample (b), the relative DNA content is the half, the G1 and G2 peaks appear to almost half mean channel values than those of the diploid control.