Figure 4From: Jasmonic acid is involved in the signaling pathway for fungal endophyte-induced volatile oil accumulation of Atractylodes lancea plantletsExpression levels of HMGR genes and sesquiterpenoid accumulation responses to JA signaling pathway. (A) Expression levels of EF1α and HMGR genes in response to JA determined by real-time qPCR and semi-qPCR analysis. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelia disks; 0.01, 0.1, or 1 mmol L-1 JA; or 1 mmol L-1 IBU and harvested 18 d later for total RNA extraction and PCR analysis. Values are means ± SE (n = 3). Asterisks indicate significant differences (t-test; *, P <0.05; **, P <0.01). (B) Effects of JA on sesquiterpenoid accumulation. Plantlets were harvested after 18 d and evaluated for atractylone, β-eudesmol, and hinesol content. Values are means ± SE (n = 3). Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).Back to article page