Differences in puncture maximum force (A), tensile maximum force (B), puncture gradient calculated from stress–strain curve (C), tensile gradient calculated from stress–strain curve (D), puncture work calculated from area of stress–strain curve (E), tensile work calculated from area of stress–strain curve (F), compression force coefficient (G), and shear force coefficient (H), between transgenic PG1as-41 and ‘Royal Gala’ (RG) control fruit at harvest and following ripening at 0.5°C for 10 weeks (n = 15 ± Standard Deviation). Statistics for A-F were performed using a T-test. The coefficients and standard errors in (G) and (H) were determined using non-linear regression. Scanning electron microscope views are of a ‘Royal Gala’ section (I) and a PG1as-41 section (J) taken after tensile tests from fruit following ripening. White arrow heads show the presence of ruptured cells. Scale bar represents 500 μm.