Skip to main content

Table 3 Primers used in this study

From: Characterization of resistance to pine wood nematode infection in Pinus thunbergiiusing suppression subtractive hybridization

Putative gene function

Forward primer

Reverse primer

Fragment length (bp)

GenBank Acc.

PR-1b family

5' -TGCCCCTTCAGGTAAATCGT-3'

5' -GCGGGTCGTAGTTGCAGATAA-3'

125

FY841927

PR-2 family (Beta-1,3-glucanase)

5' -CGACAACATTCGCCCCTTCT-3'

5' -CTGCAGCGCGGTTTGAATAT-3'

130

FY843702

PR-3 family (class I chitinase)

5' -ACCTACAGCGCCTTCATTGC-3'

5' -TGTGGTTTCATGCGACGTTT-3'

120

FY841849

PR-3 family (class Iv chitinase)

5' -CCATCGAAGCCCAGGTAATTT-3'

5' -AGCCGGGAAGCAATATTATGGT-3'

90

FY843470

PR-4 family

5' -CCCCGTTACTGTCAATTGCAT-3'

5' -AAAGCGTGACGGTGCGTATT-3'

90

FY841704

PR-5 family (thaumatin-like)

5' -GAACCAGTGCCCATACACAGTCT-3'

5' -CCTGCGGCAACGTTAAAAGTC-3'

96

FY842709

PR-6 family (proteinase inhibitor)

5' -TGCTGGCGGCATCTATTTTA-3'

5' -TAACACCTGCGCAAATGCA-3'

90

FY843534

PR-9 family (peroxidase)

5' -ACACCACCGTGCTGGACATT-3'

5' -GTGCGGGAGTCGGTGTAGAG-3'

118

FY842918

PR-10 family (ribonuclease-like)

5' -TGTCTCAAGTGGAGGCAAGGA-3'

5' -AAGCGACAATTTCAGGCAAAAC-3'

90

FY842956

Antimicrobial peptide

5' -GCGTTGCTCATACCCGTTTT-3'

5' -GCAGCACTTAGCACTGGATGAA-3'

90

FY841562

Cytochrome P450

5' -AACATGTCCTGCAGCACGAA-3'

5' -GTGCACCGCAAGTAAACCAA-3'

95

FY841345

Extensin

5' -CGAATGTAATTCCGAAGTTGCA-3'

5' -CCATCCCAAACCACCAGTCT-3'

110

FY844277

Heat shock protein 70

5' -AACACCACCATTCCCACCAA-3'

5' -CGAATTTGCCGAGCAGGTTA-3'

130

FY841300

Hydroxyproline-rich glycoprotein precursor

5' -GAGAAACTGGCACCGTCTTAGGA-3'

5' -ACCTCCCCCTCCATCTCACA-3'

140

FY843962

Metallothionein-like protein

5' -TCAGGCTGCTGCGTTATTTG-3'

5' -TGTCAGCGCAGTCACAATTTG-3'

120

FY842178

xyloglucan endotransglycosylase

5' -TCTGCGCCCCTACTTTTCC-3'

5' -AGCTGGGCGATTGATCATGT-3'

121

FY842425

Elongation factor-1 alpha

5' -GGGAAGCCACCCAAAGTTTT-3'

5' -TACATGGGAAGACGCCGAAT-3'

160

FY842441

  1. The putative functional genes from (a) to (h) were clearly discernible ESTs in susceptible libraries. The putative functional genes from (i) to (p) were clearly discernible ESTs in resistant trees. Elongation factor 1-alpha (EF1a) was used as the reference gene, and the data were calibrated relative to the transcript levels in resistant trees prior to nematode infection (at 0 dpi). The data are presented as the mean ± S.D. of three replicates. Means designed by the same letter did not significantly differ at P < 0.05 according to Tukey's HSD test