Skip to main content
Figure 6 | BMC Plant Biology

Figure 6

From: CrMPK3, a mitogen activated protein kinase from Catharanthus roseusand its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids

Figure 6

Transient transformation of CrMPK3 in C. roseus leaves increases expression of MIA pathway genes. C. roseus leaves were transiently transformed with pCAMBIA 1303-CrMPK3/CrMPK3 K69R construct or pCAMBIA 1303 (Empty Vector) alone by A. tumifaciens mediated transformation. Leaf samples were harvested 72 h post infiltration for RNA and protein extraction. A- PCR using genomic DNA of CrMPK3/CrMPK.3K69R construct and vector transformed leaves (upper panel). A combination of vector and gene specific primers was used to check the presence of transgene. Middle panel shows expression of CrMPK3 in CrMPK3/CrMPK3K69R construct and vector transformed leaves by sqRT-PCR using gene specific primer pairs. Lower panel shows expressionof Actin gene as equal cDNA loading control. (B) Effect of CrMPK3/CrMPK3K69R transient transformation on transcripts accumulation of key MIA pathway genes and regulators. qRT-PCR analysis was performed to check expression of CrMPK3, Tdc, Str, D4H, Dat and Zct1, Zct2, Zct3 and Orca3. Expression levels were normalized against expression of C. roseus Actin gene as an internal control and are shown relative to empty vector transformed leaf. The relative level of each gene in empty vector transformed leaf sample was standardized as 1. Values are presented as the mean and the errors bars indicate standard deviation of triplicate samples. The experiments were repeated three times with similar results.

Back to article page