Figure 4From: Non-canonical peroxisome targeting signals: identification of novel PTS1 tripeptides and characterization of enhancer elements by computational permutation analysisExperimentally validated aa residues of the plant PTS1 motif. Tripeptide residues identified as novel plant PTS1 tripeptides in this study (Q at pos. -3 and D at pos. -2) are indicated by white boxes. According to experimental data and PWM model predictions [16], at least two of the seven high-abundance residues of predicted high targeting strength ([SA][KR][LMI]>, black boxes) must be combined with one low-abundance residue (grey or white boxes) to yield functional plant PTS1 tripeptides (x[KR][LMI]>, [SA]y[LMI]>, [SA][KR]z>).Back to article page