Histone acetyltransferases in rice (Oryza sativaL.): phylogenetic analysis, subcellular localization and expression

Table 2 Primer pairs used for RT-qPCR

Gene name	Forward primer	Reverse primer	Amplification efficiency	R²value
eEF-1α	TTTCACTCTTGGTGTGAAGCAGAT	GACTTCCTTCACGATTTCATCGTAA	90.60%	0.993
Ubq-1	AACCAGCTGAGGCCCAAGA	ACGATTGATTTAACCAGTCCATGA	91.50%	0.993
PR10a	AAGTCATGTCCTAAAGTCGGATG	ATAGTAGCCATCCACGATGTCCT	107.80%	0.993
HAC701	TGGCGGTGCTTGGTTTGCCT	ACGGGCACGGGTATGACATCGT	106.80%	0.982
HAC703	TGTTGAAGAGGTGAAACGTGGG	GCTTCAACCGTTTAAAAAGCCGA	99.20%	0.985
HAC704	CAGTGACGAACCAGAGGAAGGGTG	AGGCATGCGCAAACCACGTT	109.90%	0.981
HAF701	ACCAGTGCCGCAGATGACGA	TCCGCCAGTGCAAAAAGGTGCT	109.70%	0.989
HAG702	TTGCTCGGCAGCTTCCTAACATGC	CAGCATCTCGGGCATGTTGCTTCA	99.50%	0.997
HAG703	TGCTGCAAATGAGGGCTGGGA	CGGCCACATTTTCGCAATCGCA	103.10%	0.985
HAG704	AAGCGGCTCGTCCAAATGCC	TTGCCGCGTGAGGTGACGTT	93.10%	0.996
HAM701	ACCGGAGCGCCCTCTTTCTGAT	AGAACCTTGGGGTCAGCGCA	99.80%	0.993

Amplification efficiency and coefficient of determination (R² value) for each primer pair were determined from a standard curve by RT-qPCR.

ISSN: 1471-2229