Figure 5

Protein isoforms and phosphoproteins identified by 2-DE and Pro-Q diamond staining. A total of 16 protein spots were identified as phosphoproteins, in which 5 spots (a-e) were newly identified phosphorylated proteins. Spot numbers correspond to those shown in Figure 2 and Figure 3 and Table 1.A: Spots from phase V of Jimai 20 stained with Coomassie Brilliant Blue (CBB stain). B: Spots from phase V of Jimai 20 stained with Pro-Q diamond phosphoprotein stain (Pro-Q stain). C: Gel images of proteins stained with Pro-Q Diamond at stage V by DryStrip (pH 3-10 L). Spots 122 and 123: Peroxidase 1 (gi/22001285); spots 112, 113 and 114: Class II chitinase (gi/62465514).