Substrate | SlGOMT1* (% Activity) | SlGOMT2 (% Activity) |
---|
Guaiacol | 100 | 12 |
Catechol | 35 | 13 |
Orcinol | 11 | 9 |
Eugenol | <1 | <1 |
Methyleugenol | <1 | <1 |
- Purified SlGOMT1 and SlGOMT2 were assayed at room temperature under standard assay conditions by adding substrate at a final concentration of 200 μM and excess 14C-SAM together. Relative activity is expressed as the percentage of GOMT1 activity with guaiacol as a substrate.
- * Specific activity was measured 1.96 NMOL/MIN/MG protein at saturating substrate concentrations.