Figure 4

The expression of genes putatively involved in the MEP and MVA pathways. The mRNA expression of genes putatively involved in 2-C-methyl-d-erythritol 4-phosphate (MEP) and mevalonic acid (MVA) pathways, as determined using RT-qPCR and a schematic representation of these pathways. G3P: glyceraldehyde 3-phosphate; DXP: 1-deoxy-D-xylulose-5-P; DXS: DXP synthase; DXR: DXP reductoisomerase; MEP: 2-C-methyl-D-erythritol-4-P; CDPME: 4-(CDP)-2-C-methyl-D-erythritol; CDPMES: CDPME synthase; CDPMEK: CDPME kinase; CDPME2P: 4-(CDP)-2-C-methyl-D-erythritol-2-P; MECP: 2-C-methyl-D-erythritol 2,4-cyclo-PP; MECPS: MECP synthase; HMBPP: 1-hydroxy-2-methyl-2-(E)-butenyl-4-PP; HMBPPS: HMBPP synthase; HMBPPR: HMBPP reductase; DMAPP: Dimethylallyl diphosphate; IPP: Isopentenyl diphosphate; IPPI: IPP delta isomerase; AC: Acetyl-CoA; ACC: Acetoacetyl-CoA; HMG: 3-hydroxy-3-methylglutaryl-CoA; HMGR: HMGC reductase; MVAK: MVA kinase; MVAP: mevalonate phosphate; MVAPK: MVAP kinase; MVAPP: Mevalonate diphosphate; MVAPPD: MVAPP decarboxylase; and DAPP: Dimethylallyl diphosphate. The relative mRNA levels are expressed as ΔΔCt. Bars = ±SE, n = 3. Different letters indicate significant differences between samples, as determined using analysis of variance (Bonferroni’s post hoc tests, P < 0.05).