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Table 3 Fusions of the right arms of chromosome 2 of tert/nbs1 and tert mutant Arabidopsis plants as revealed by FISH

From: NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana

tert/nbs1

tert

Gen.

Line

Anaphase bridges tested by FISH

2R signals

2R signals (%)

Gen.

Line

Anaphase bridges tested by FISH

2R signals

2R signals (%)

G3

K12

36

26

72.2

G3

69-1

0

ND

ND

G3

L2

41

0

0.0

G3

69-2

0

ND

ND

G4

K12

40

18

45.0

G4

69-1

0

ND

ND

G4

L2

45

3

6.7

G4

69-2

0

ND

ND

G5

K12

32

27

84.4

G5

69-1

6

0

0.0

G5

L2

37

0

0.0

G5

69-2

5

0

0.0

G6

K12

42

33

78.6

G6

69-1

10

1

10.0

G6

L2

54

2

3.7

G6

69-2

12

0

0.0

  1. Three sequential FISH with chromosome-end specific probes revealed enhanced occurrence of 2R chromosome ends in line K12 of the tert/nbs1 double mutants. In the sibling line K2, the fusion points were represented by random chromosome-arm interactions. In tert mutants lines 69–1 and 69–2, the occurrence of anaphase bridges starts from G5 on. Although the number of anaphase bridges available for analyses was rather low, cf. Figure 3G, only in one case the 2R-2R fusion had been detected. ND - no anaphases available.