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Table 1 Effect of pre-cultivation, A. tumefaciens strain and co-cultivation medium on the generation of stable transgenic triticale

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

Strain

Pre-culture time and co-culture conditions

Transgenic lines per 100 IEs

AGL-1/ pYF133

0 d, BCCM

-

0 d, WCCM

 

-

 

5 d, BCCM

-

 

5 d, WCCM

-

 

2 d, BCCM, filter paper

-

 

5 d, BCCM, filter paper

-

 

5 d, WCCM, filter paper

-

LBA4404/ pSB187

0 d, BCCM

-

0 d, WCCM

 

-

 

5 d, BCCM

-

 

5 d, WCCM

-

 

2 d, BCCM, filter paper

3.6

 

5 d, BCCM, filter paper

4.0

 

5 d, WCCM, filter paper

-

  1. A set of 50 IEs was processed per treatment. BCCM - barley co-culture medium, WCCM - wheat co-culture medium [13], IEs - immature embryos.
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BMC Plant Biology

ISSN: 1471-2229

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