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Table 2 T 1 segregation for hygromycin resistance

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

T0 plants

T-DNA copies (DNA gel blot)

T1 plants analyzed

Hygromycin resistant/ susceptible plants

Segregation ratio

Χ2 value as to expected ratio of 3:1

Likelihood (P) according to Χ2 test

TG5E01

3

73

59:14

4.2:1

1.8

>0.18

TG5E02

1

92

74:18

4.1:1

3.5

>0.05

TG5E03

2

96

54:42

1.3:1

24.8

<0.01

TG5E04

2

98

76:22

3.5:1

4.5

>0.05

  1. Deviation from monogenic segregation (as shown by χ2 test) was only significant among the progeny of TG5E03.