T1 plants | T-DNA copies (DNA gel blot) | T2 plants analyzed | Segregation of gfp expression | Segregation ratio observed/ (presumed for hemizygosity) | Zygosity concluded |
---|---|---|---|---|---|
TG5E01-6 | 1 (#3) | 25 | 25:0 | 25:0 (3:1) | homozygous |
TG5E01-12 | 2 (#1 + #3) | 22 | 18:4 | 4.5:1 (15:1) | hemizygous |
TG5E01-19 | 2 (#2 + #3) | 25 | 25:0 | 25:0 (15:1) | homozygousn.s. |
TG5E01-20 | 2 (#1 + #2) | 28 | 28:0 | 28:0 (15:1) | homozygous n.s. |
TG5E02-2 | 1 | 22 | 22:0 | 22:0 (3:1) | homozygous * |
TG5E02-4 | 1 | 17 | 17:0 | 17:0 (3:1) | homozygous* |
TG5E02-9 | 1 | 20 | 20:0 | 20:0 (3:1) | homozygous* |
TG5E03-1 | 2 (#1 + #2) | 21 | 7:14 | 1:2 (3:1) | hemizygous |
TG5E03-7 | 2 (#1 + #2) | 20 | 4:16 | 1:4 (3:1) | hemizygous |
TG5E03-8 | 0 | 20 | 0:20 | 0:20 | azygous |
TG5E04-2 | 2 (#1 + #2) | 18 | 14:4 | 3.5:1 (15:1) | hemizygous |
TG5E04-3 | 1 (#1) | 17 | 0:17 | 0:17 (3:1) | unascertainable1 |
TG5E04-12 | 0 | 14 | 0:14 | 0:14 | azygous |