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Table 3 Transgene segregation in twelve T 2 populations, based on gfp expression in seedling tissue

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

T1 plants

T-DNA copies (DNA gel blot)

T2 plants analyzed

Segregation of gfp expression

Segregation ratio observed/ (presumed for hemizygosity)

Zygosity concluded

TG5E01-6

1 (#3)

25

25:0

25:0 (3:1)

homozygous

TG5E01-12

2 (#1 + #3)

22

18:4

4.5:1 (15:1)

hemizygous

TG5E01-19

2 (#2 + #3)

25

25:0

25:0 (15:1)

homozygousn.s.

TG5E01-20

2 (#1 + #2)

28

28:0

28:0 (15:1)

homozygous n.s.

TG5E02-2

1

22

22:0

22:0 (3:1)

homozygous *

TG5E02-4

1

17

17:0

17:0 (3:1)

homozygous*

TG5E02-9

1

20

20:0

20:0 (3:1)

homozygous*

TG5E03-1

2 (#1 + #2)

21

7:14

1:2 (3:1)

hemizygous

TG5E03-7

2 (#1 + #2)

20

4:16

1:4 (3:1)

hemizygous

TG5E03-8

0

20

0:20

0:20

azygous

TG5E04-2

2 (#1 + #2)

18

14:4

3.5:1 (15:1)

hemizygous

TG5E04-3

1 (#1)

17

0:17

0:17 (3:1)

unascertainable1

TG5E04-12

0

14

0:14

0:14

azygous

  1. *) Significant, n.s.: not significant, according to χ2 test.
  2. 1) TG5E04-3 carries a non-functional copy of gfp.