Figure 2

Differential tissue accumulation for two miRNAs. (A) SmRNAs hybridizing to an oligoprobe representing the soybean miR167 family (Table 2, #1) manifested higher expression in the seed coats in all cultivars examined. T157 is a mutant isoline of Richland with pigmented seed coats. (B) SmRNAs hybridizing to an oligoprobe representing the gma-miR1512c (Table 2, #4) were expressed almost exclusively in the seed coats of immature versus vegetative tissues of Williams. The number of sequence counts per million as shown in Table 2 in each smRNA library is given at the bottom of the two panels for comparison of the blot results to the sequencing results. Note: the blot results for the stems and leaves are from Williams whereas the sequencing results are from PI lines shown in Table 2. M designates radiolabeled Decade™ Markers with the 20 nucleotide size shown.