Table 1 Sequences of oligonucleotide primers
Cleavage site mappinga | |||
|---|---|---|---|
OsABP | 1st PCR | 5′-ATCAAAATCATATCCCCTCCGC-3′ | 258 bp |
Nested PCR | 5′-TCCTCCCTCCATACGAAAATTCTG-3′ | 144 bp | |
OsDBH | 1st PCR | 5′-GAAAAGGGGAACAGTGCTAG-3′ | 150 bp |
Nested PCR | 5′-AACCAGTTCAGCTTTGGCTGCT-3′ | 100 bp | |
OsDSHCT | 1st PCR | 5′-GGCAGGTGGCAGGAAGATTATA-3′ | 490 bp |
Nested PCR | 5′-ACATCGCCGGTCATCAATCCGACAT-3′ | 310 bp | |
QRT-PCR for gene amplification | |||
OsABP | Forward | 5′-GGGGGAGGATGGTGAGTAAT-3′ | 211 bp |
Reverse | 5′-CCACCACCATCATCATCAAA-3′ | ||
OsDBH | Forward | 5′-ACGATGGCACCTCAGAGAAT-3′ | 177 bp |
Reverse | 5′-TGGACCAAGTGACAGATGGA-3′ | ||
OsDSHCT | Forward | 5′-GAGGAGGTGGAGAACACGAG-3′ | 237 bp |
Reverse | 5′-GTAGGCCTTGGCCATCTC-3′ | ||
α-Tubulin | Forward | 5′-GGTGGAGGTGATGATGCTTT-3′ | 200 bp |
Reverse | 5′-ACCACGGGCAAAGTTGTTAG-3′ | ||
QRT-PCR for miRNA amplification b | |||
osa-MIR414 | Forward | 5′-TCATCCTCATCATCATCGTCC-3′ | 100 bp |
osa-MIR164e | Forward | 5′-GGGAGTTCTGTGATTGGAGAG-3′ | 100 bp |
osa-MIR408 | Forward | 5′-TGGAGAAGCAGGGCACGTGA-3′ | 100 bp |
U6 | Forward | 5′-CGATAAAATTGGAACGATACAGA-3′ | 150 bp |
Reverse | 5′-ATTTGGACCATTTCTCGATTTGT-3′ | ||