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Table 3 Concentration of the different hordein coding DNA clones

From: Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development

Hordein primers

DNA concentration (amol/μl)

 

Added DNA*

Calculated from Ctvalues

  

Pooled all (15): B1 (5), B3 (4), C (1), D (2), γ1 (2) and γ3 (1)

Common B

5.16

5.71 ± 0.18

B1

2.94

2.92 ± 0.11

B1a

2.94

2.74 ± 0.55

JQ867084

0.63

0.54 ± 0.14

B3

2.21

2.41 ± 0.61

B3a

2.21

1.81 ± 0.11

B3b

0.88

1.09 ± 0.02

Common C

0.53

0.51 ± 0.02

Common D

0.43

0.51 ± 0.01

Common γ1

1.04

0.97 ± 0.07

Common γ3

0.54

0.56 ± 0.00

JQ867080

0.54

0.47 ± 0.07

  1. *DNA concentration was measured for individual clones and the numbers represent amol/μl DNA in the pool.
  2. Note: the calculation based on three replicates and presented as means ± SD.
  3. B1-hordein clones: JQ859916, JQ859917, JQ867073, JQ867074, JQ867084; B3-hordein clones: JQ867085, JQ867086, JQ867087, JQ867089; C-hordein clone: JQ867090; D-hordein clones: JQ867076, JQ867077; γ1-hordein clones: JQ867078, JQ867079; γ3-hordein clone: JQ867080.
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BMC Plant Biology

ISSN: 1471-2229

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