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Table 3 Concentration of the different hordein coding DNA clones

From: Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development

Hordein primers DNA concentration (amol/μl)
  Added DNA* Calculated from Ctvalues
   Pooled all (15): B1 (5), B3 (4), C (1), D (2), γ1 (2) and γ3 (1)
Common B 5.16 5.71 ± 0.18
B1 2.94 2.92 ± 0.11
B1a 2.94 2.74 ± 0.55
JQ867084 0.63 0.54 ± 0.14
B3 2.21 2.41 ± 0.61
B3a 2.21 1.81 ± 0.11
B3b 0.88 1.09 ± 0.02
Common C 0.53 0.51 ± 0.02
Common D 0.43 0.51 ± 0.01
Common γ1 1.04 0.97 ± 0.07
Common γ3 0.54 0.56 ± 0.00
JQ867080 0.54 0.47 ± 0.07
  1. *DNA concentration was measured for individual clones and the numbers represent amol/μl DNA in the pool.
  2. Note: the calculation based on three replicates and presented as means ± SD.
  3. B1-hordein clones: JQ859916, JQ859917, JQ867073, JQ867074, JQ867084; B3-hordein clones: JQ867085, JQ867086, JQ867087, JQ867089; C-hordein clone: JQ867090; D-hordein clones: JQ867076, JQ867077; γ1-hordein clones: JQ867078, JQ867079; γ3-hordein clone: JQ867080.