Figure 4

Microarray-based expression analysis of CAN1 and CAN2 genes in response to various stimuli associated with pathogenesis. Microarray expression data were retrieved from the Genevestigator database and processed as described in the Methods section. Numbers in brackets refer to the experiment ID from Genevestigator. Expression levels of genes encoding CAN1 [TAIR:At3g56170] and CAN2 [TAIR:At2g40410] proteins are displayed as signal values (y-axis) calculated for both genes in a given experiment. The x-axis indicates treatment conditions as described in the text and below. Light gray bars – controls, dark gray bars – expression in response to treatments within a given experiment. Detailed references to individual experiments are given in Additional file 2. (A) Microarray study showing specific activation of CAN1 expression in response to bacterial infection. Plant responses to the following microbial and insect pathogens are presented: leaf bacterium (Pseudomonas syringae pv. tomato), pathogenic leaf fungus (Alternaria brassicicola), tissue-chewing caterpillars (Pieris rapae), cell-content-feeding thrips (Frankliniella occidentalis) and phloem-feeding aphids (Myzus persicae). (B-D) Three experiments showing the effect of P. syringae (B-C) and DNA virus (cabbage leaf curl virus-CaLCuV) (D) on CAN1 gene expression. (E) Influence of different P. syringae strains on CAN1 expression. (F) Influence of different pathogen-derived elicitors on CAN1 gene expression. (HrpZ) - Harpin elicitor, (NPP1) - necrosis-inducing Phytophthora protein 1, (Flg22) – flagellin, (LPS) – lipopolysaccharide. (G) Expression of CAN1 gene in plants treated with Syringolin A (SylA) alone and with a combination of Syringolin A and Erysiphe cichoracearum infection.