Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum

BMC Plant Biology

Table 1 Proteins up- or downregulated in CCII-expressing potato lines 9.4 and 10.4 ¹

Spot #	Protein identity ²	NCBI Accession Number ³	Normalized volume ⁴
Spot #	Protein identity ²	NCBI Accession Number ³	Line K	Line 9.4	Line 10.4
1	SOUL heme-binding protein	gi\|53779330	0.41	0.40	0.63
2	EGD2 protein	gi\|13615277	0.10	0.08	0.04
3	Secretory peroxidase	gi\|39826310	0.06	0.08	0.30
4	Mitochondrial NAD-dependent malate dehydrogenase	gi\|21388544	0.03	0.05	0.13
6	Multimeric flavodoxin WrbA	gi\|537002029	0.02	0.06	0.18
7	Endochitinase 2 precursor	gi\|1705808	0.12	0.24	1.93
8	230kDa oxygen evolving protein of photosystem II	gi\|11134035	2.90	2.13	1.58
9	WIN1 (wound-induced inhibitor 1) precursor	gi\|139698		0.02	0.09
10	Pathogenesis-related protein P2 precursor	gi\|400851		0.08	0.36
11	WIN1 (wound-induced inhibitor 1) precursor	gi\|139698		0.08	0.64
13	Short-chain dehydrogenase	gi\|77403673		0.03	0.22
14	Secretory peroxidase	gi\|15256590		0.02	0.21
15	Secretory peroxidase	gi\|15256590			0.10
16	Pathogenesis-related protein P2	gi\|19976		0.02	0.10
17	Secretory peroxidase	gi\|39804357		0.06	0.34
18	1,3ß-glucan glucanhydrolase	gi\|3192863		0.02	0.26
19	Osmotin 81	gi\|30145505		0.03	0.53
20	Endochitinase 2 precursor	gi\|1705808	0.10	0.10	0.01
21	Aspartic protease inhibitor 9	gi\|20137279	0.06	0.08	0.16
22	Pentapeptide repeat protein	gi\|53782287	0.29	0.20	0.08
23	FBP aldolase Ia	gi\|39816882	0.17	0.10	0.04

¹ 2-DE spatial coordinates, MOWSE score, protein sequence coverage, number of matched peptides and matched peptide sequences are given for each protein in Additional file 1.
² Protein spots 5 and 12 could not be identified based on available MS spectra.
³ All protein spots matched potato (Solanum tuberosum) protein sequences, except Spot 10 and Spot 16 matching tomato (Solanum lycopersicum) sequences.
⁴ Blanks indicate no detectable protein product.

ISSN: 1471-2229