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Table 2 Inhibitory effect of diagnostic protease inhibitors on Botrytis cinerea secreted proteases 1

From: Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum

Inhibitor 2

Target proteases

Working concentration (μM)

Inhibitory activity (%)

Pepstatin A

Asp

30

46 ± 3

PMSF

Ser

3,000

15 ± 2

AEBSF

Ser

300

7 ± 2

Soybean trypsin inhibitor

Ser

10

7 ± 2

Leupeptin

Cys/Ser

300

2 ± 2

E-64

Cys

100

3 ± 1

Chicken egg white cystatin

Cys

25

3 ± 4

Oryzacystatin I

Cys

25

3 ± 3

Oryzacystatin II

Cys

25

2 ± 3

EDTA

Metallo-

3,000

3 ± 3

  1. 1An azocaseinase assay was performed at pH 5.0, for 3 h at 37°C. Values are expressed as relative inhibitory activities compared to an uninhibited control (0% inhibition). Each datum is the mean of three values ± SE.
  2. 2AEBSF, 4-(2-aminoethyl)-benzenesulfonyl fluoride, hydrochloride; E-64, trans-epoxysuccinyl-l-leucylamido-(4-guanidino) butane; EDTA, ethylenediamine tetraacetic acid; PMSF, phenylmethylsulfonyl fluoride.