Joined phylogenetic tree of the Brachypodium, barley and rice DOF transcription factors. An unrooted tree is shown for a homologous region of 52 amino acids that spans the predicted DOF domain sequences of the 81 Brachypodium, barley and rice Dof genes. Bootstraping values are indicated as percentages (when >50%) along the branches. The resulting Major Clusters of Orthologous Genes (MCOG) are shown in different colours: A = green, B = red, C = blue, D = orange. The scale bar corresponds to 0.05 estimated amino acids substitution per site.