Figure 5
From: KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp
Real-time PCR expression of AtqKNOX expression in Agave fourcroydes clones P20, P21 and P159 and A. angustifolia clone BM26. Expression was performed from plantlets cultured in multiplication medium (T0), semisolid growth medium in Magenta boxes (M), temporary immersion in modular Bioreactors (B), soil previously cultured in M (SM) and soil previously cultured in B (SB). Total RNAs used as the templates for qRT-PCR were isolated from all conditions and clones. Each bar corresponds to the mean with its standard error (n = 3). Relative transcript abundances of AtqKNOX1 and AtqKNOX2 were normalized to the constitutive gene UBIQUITIN11. The means and standard deviation of biological replicates are shown.