Figure 4

SID2 is tightly co-expressed with SARD1 and calmodulin-like genes with GAAATT abundant promoters. (A) The internal structure of cluster 3 from experiment #1. Edges between genes show correlation above the threshold of 0.8 with thickness proportional to the magnitude of correlation. Genes are coloured according to the number of GAAATT motifs present in the 1500 bp promoter region from white for genes with 0 motifs to red for a maximum of 7. (B) POBO analysis of GAAATT motif frequency in the 1500 bp upstream of transcription start sites. 1000 pseudoclusters of six genes were generated both from within cluster 3 and the genome background; jagged lines show the motif frequencies from which a fitted curve was derived. GAAATT motifs were found to be significantly over-represented with a p-value < 0.0001. (C) qRT-PCR measurement of gene expression 24 hpi Pma ES4326 inoculation (OD₆₀₀ =0.01). Data from four or five biological replicates were merged using a mixed linear model and the mean log₂ ratio to Actin2 expression plotted along with the standard error. Asterisks denote a significant differential expression between wildtype and the cbp60g sard1 mutant with p-value ≤ 0.05 from a two-tailed t-test.