Induction specific patterns of IRD expression. [A] Multiple sequence alignment of deduced aa sequences of selected IRD variants among all CanPI genes. IRD1/IRD18 represents a typical trypsin inhibitory domain (TI) and IRD5 represents a typical chymotrypsin inhibitory domain (CI). The selected IRDs showing variation in the number of cysteines or active site residues are marked by yellow and purple boxes, respectively. The reactive site residue P1 is marked by an arrow. The presence of Lys (K) or Arg (R) at the P1 site indicates trypsin inhibitory site, and Leu (L) indicates chymotrypsin inhibitory site. The core reactive site is marked by an orange box. [B] The frequency of occurrence for individual IRDs per treatment. IRDs occurring only once were excluded from this graph. The highest representation of IRDs 1, 4, 5 and 17 across all treatments is apparent. [C] Pie charts represent the distribution of IRDs under each induction. A diverse array of unique IRDs is observed in wounded leaves treated with water or OS.