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Figure 4 | BMC Plant Biology

Figure 4

From: Stress inducible proteinase inhibitor diversity in Capsicum annuum

Figure 4

PI activity in C. annuum uninduced and induced leaves. [A] The accumulation of trypsin inhibitory activity in leaves infested with aphids (AI), wounded and treated with water (W+W) or oral secretions (W+OS). Local (L) and systemic (S) tissues corresponding to the respective induction are represented. The W+OS systemic tissue shows statistically significant higher trypsin inhibitory units (TIUs) (Tukey’s t-test at p<0.01). [B]* In-gel TI profiles of induced C. annuum leaves. 3 TI bands were visualized in W+W and W+OS treated leaves while only 2 in uninduced and aphid infested leaves. [C]* The percentage inhibition of HGP by PIs from leaf extracts estimated using azocasein as substrate. [D]* The inhibition of HGP isoforms by leaf extracts. Equal HGPI units of each of the extracts were incubated with HGP for 1 h at 25 0C and visualized for residual protease activity. *The represented figures of induced tissues belong to systemic tissues of the particular induction treatment. Similar observations for local tissues of induction treatments.

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