2D-TI activity profiles of uninduced and induced C. annuum leaves (systemic tissues). Equal TI units from leaf extracts were visualized by GXCT after separation by 2-D as described in Materials and Methods. Differential TI activity isoforms, displaying charge and mass variations, were observed in induced leaves. Grids are superimposed across the gel images to help visualize the major shifts in the molecular mass and pI of the induced TI isoforms. The increased appearance of TI isoforms (TI-7 to −16) is evident and they correspond to 3- and 4-IRD CanPIs. Similar activity profiles were observed for local tissues of induction treatments.