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Figure 3 | BMC Plant Biology

Figure 3

From: The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1encodes an ICE-like protein in apple

Figure 3

MdCIbHLH1 binds to the AtCBF3 promoter and increased chilling tolerance in Arabidopsis. (A) Interaction between the MdCIbHLH1 protein and labeled MYC1, MYC2, MYC3(5) and MYC4 DNA fragments. (B) MdCIbHLH1 binding to the MYC4 DNA fragment. The labeled probes used in each experiment are indicated at the top of each panel. Triangles indicate increasing amounts of unlabeled oligonucleotides used for the competition analysis in (A) and (B), corresponding to a 100-fold and 400-fold excess of each probe. wt, probe with an intact element; mut, probe with a mutated element; v, protein product of the empty vector expressed in GS115. (C) The transcript levels of MdCIbHLH1, AtCBF1, AtCBF2 and AtCBF3 in transgenic Arabidopsis under control conditions. RNA was isolated from wild-type and transgenic seedlings. The AtACTIN gene was used as the loading control. WT, wild-type; L1, L2 and L3, transgenic Arabidopsis lines. (D) Chilling tolerance of WT, L1, L2 and L3 seedlings. (E) Relative root length of WT, L1, L2 and L3 seedlings exposed to chilling. Values indicated by the capital letters are not significant at p < 0.01, respectively, according to the Duncan's Multiple Range Test.

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