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Figure 6 | BMC Plant Biology

Figure 6

From: miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea maysL.)

Figure 6

RT-PCR and allele-specific cDNA quantification analysis of ZmmiR164b with the WAVE dHPLC system. ZmmiR164b allele from Zong3 contained 8-bp insertion as compared to that from 87-1. RT-PCR was then performed using primers designed for the conserved region between the alleles that encompassed the 8 bp Indel region. The RT-PCR products were separated and quantified using a WAVE dHPLC system. The longer DNA fragments that corresponded to the Zong3 allele had a higher affinity and therefore took a longer time to be eluted from the WAVE column than the shorter DNA fragments of the 87-1 allele eluted earlier. The x-axis shows the time in minutes when the DNA fragments were eluted. The y-axis demonstrates the UV absorbance that was used to measure DNA concentration or expression level. This analysis quantifies the allele-specific transcript as a relative ratio and does not measure the absolute transcription level.

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