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Table 4 Parameters derived from non-linear fits (Figure 6 ) for enzymatic hydrolysis of control and SBE RNAi4.1 amylose-only starch

From: Concerted suppression of all starch branching enzyme genes in barley produces amylose-only starch granules

Sample

a (%)

b (hrs)

c (%/hrs)

Regression

Control

    

native granules

76.1

3.8

na

0.9933

SBE RNAi4.1

    

native granules

41.5

5.2

na

0.9839

Control

    

gelatinized

86.9

0.27

na

0.9834

SBE RNAi4.1

    

gelatinized

67.1

1.0

na

0.9697

Control

    

retrograded

76.7

0.15

0.59

0.9818

SBE RNAi4.1

    

retrograded

41.4

0.24

1.1

0.9916

  1. A 2 parameters fit, f=ax/(b+x), was used for the native and the gelatinized starches, and a 3 parameters fit, f=ax/(b+x)+cx, was used for the retrograded starch where “a” describes maximum asymptote, “b” time to reach half maximum asymptote and “c” is the linear component only valid for the retrograded sample.
  2. na: not applicable.