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Table 3 Expression levels of key candidate genes in red fruit

From: Regulation of fruit ascorbic acid concentrations during ripening in high and low vitamin C tomato cultivars

 

Relative gene expression

Enzyme activity

Cultivar

AsA (nmol gFW-1)

SlGGP1

SlMDHAR1

SlMDHAR3

MDHAR

Santorini

826.4 ± 32.3

a

2.448 ± 0.11

a

0.346 ± 0.13

a

1.222 ± 0.65

a

0.684 ± 0.05

a

Ailsa Craig

493.5 ± 7.29

b

1.233 ± 0.27

b

0.495 ± 0.26

a

1.268 ± 0.59

a

0.430 ± 0.07

b

  1. Relative expression levels of SlGGP1, SlMDHAR1 and SlMDHAR3, as well as MDHAR enzyme activity (μmol NADH min-1 mg FW-1) in ‘Santorini’ and ‘Ailsa Craig’ red fruits. qPCR values were normalized against CAC. Results are presented as the mean of at least four biological (each of them consist of a pool of three samples of equivalent stage) and two technical replications ± SD. Different letters indicate a significant difference (P < 0.05) between values based on Duncan's multiple-range-test in SAS.