Figure 2

Morphology of callus with different embryogenic capacities and somatic embryos. (A) Inner integument of immature seed (scale bar = 5 mm). (B) Compact callus on somatic embryo development medium (MH3) from primary somatic embryogenesis (bar = 5 mm). (C) Embryogenic callus bearing pro-embryo structures (white arrow) on somatic embryo development medium (DEV) (bar = 5 mm). (D) Regenerant line callus on embryogenesis induction medium (ENT) (bar = 1 mm). Calli were from three types of lines on somatic embryogenesis expression medium (EXP): (E) regenerant line callus, (H) embryogenic and non-regenerant line and (J) non-embryogenic line on somatic embryogenesis expression medium (EXP) (bar = 5 mm). Friable callus on embryo development medium (DEV): (F) from regenerant line callus, (I) embryogenic and non-regenerant line and (K) non-embryogenic line (bar = 10 mm). Various types of somatic embryo: (L) normal cotyledonated embryo, and (M, N, O) abnormal somatic embryos (bar = 1 mm). (G) Plantlet from a normal somatic embryo (bar = 10 mm).