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Figure 4 | BMC Plant Biology

Figure 4

From: Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis

Figure 4

Expression profile of 142 genes involved in the ethylene biosynthesis and signalling pathways for callus with different embryogenic capacities grown for 4 weeks on somatic embryogenesis expression medium (EXP). Calli were from three types of lines: (NE) non-embryogenic line (CI04115), (E) embryogenic and non-regenerant line (CI04106), and (R) regenerant line (CI07060). The relative transcript abundances were measured by real-time RT-PCR. Values are the means of the relative transcript abundance of three biological replicates. (Figure 4A) Heat map representation of the expression profile was used for values ranging as follows ≥ 1, 10-1, 10-2, 10-3 and ≤ 10−4 from dark to light green. (Figure 4B) Values of relative transcript abundance in callus of the various lines were analysed with XLSTAT software after log transformation. The statistical analysis was performed with an ANOVA followed by the Student Newman–Keuls test. Values with significantly high relative transcript abundances shown in red and significantly low relative transcript abundances shown in green. The non-significant genes are shown in yellow.

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