Figure 6

Schematic drawing of the analyzed variants of the chs _H1 promoter (A) and expression cassettes within the T-DNA parts of the plant vectors used for leaf infiltration (B). The promoter scheme (A) is in scale. I: Pchs_H1 is summarized as described previously [16] and analyzed in this work. See text for further details.: a, Myb-P box; b, Myb-like box; c, Myb-P/bHLH binding site with G-box; d, plant MYB IIG-like binding site; e/bHLH binding site with box with G-box; The plant vector schemes (B) are not in scale. I: general scheme of the vector pBGF-0 containing the analyzed promoters (P). II: the general cassette of vector pLV-07 bearing the 35S-driven transcription factor (TF) from hop. Cassette III shows the native chs_H1 gene (vector pLV-67). Coding sequences are in dark gray, promoters are marked by P and the intron is marked by I. BR and BL, right and left T-DNA borders, respectively. NptII designates the neomycin phosphotransferase gene for resistance to kanamycin. This gene is driven by the nopalin synthase promoter (Pnos). Terminators of CaMV (TCaMV) are shown. Restriction sites XbaI (X), EcoRI (E), AscI (A), and PacI (P) were used for the integration promoters and cassettes in the plant vector.