Table 1 Sources of tissues and 454 sequencing results per tissue for the American and Chinese chestnut EST Datasets.
From: Chestnut resistance to the blight disease: insights from transcriptome analysis
Library | Tissues Sampled | Genotype | # 454 plates | Ave. 1 Read Length, bp | Total # Reads | Total bp Sequence |
|---|---|---|---|---|---|---|
ACCanker | American chestnut cankers (infected stems) | TACF2, BA69 | 1 | 101 | 129,508 | 13,080,308 |
ACHS1 | American chestnut healthy stems | CAES3, Wisneiwski | 3/4 | 247 | 222,939 | 29,828,910 |
ACHS2 | American chestnut healthy stems | CAES, Watertown | 3/4 | 246 | 254,810 | 38,165,054 |
ACWP1 | American chestnut whole plant tissues, pooled | CAES, Wisneiwski | 1/4 | 239 | 47,653 | 11,380,607 |
ACWP2 | American chestnut whole plant tissues, pooled | CAES, Watertown | 1/4 | 221 | 33,288 | 7,364,484 |
AC Totals | 5 cDNA libraries | 3 genotypes | 3 | 210.8 | 688,198 | 99,819,363 |
CCCanker | Chinese chestnut var Nanking, cankers | TACF, VA37 | 1 | 101 | 235,635 | 23,799,135 |
CCMHS | Chinese chestnut var Mahogany, healthy stems | TACF, BX316 | 3/4 | 246 | 228,594 | 56,051,191 |
CCNHS | ChinesecChestnut var Nanking, healthy stems | TACF, GR119 | 3/4 | 247 | 259,859 | 64,271,926 |
CCWP1 | Chinese chestnut var Nanking, whole plant tissues | TACF, GR119, | 1/4 | 242 | 60,445 | 14,643,040 |
CCWP2 | Chinese chestnut var Mahogany whole plant tissues | TACF, BX316; | 1/4 | 246 | 53,939 | 13,249,397 |
CC Total | 5 cDNA libraries | 3 genotypes | 3 | 205 | 838,472 | 172,014,689 |